Saccharin Supplementation Inhibits Bacterial Growth and Reduces Experimental Colitis in Mice.

ABSTRACT: Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis. Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice. In vitro, different bacterial strains were grown in the presence or absence of saccharin. Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed. Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative

SUBMITTER: Sunderhauf A 

PROVIDER: S-EPMC7230785 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature