The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli.

ABSTRACT: A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indi

SUBMITTER: Krause K 

PROVIDER: S-EPMC7231150 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature