Metabolic Switching of Tumor Cells under Hypoxic Conditions in a Tumor-on-a-chip Model.

ABSTRACT: Hypoxia switches the metabolism of tumor cells and induces drug resistance. Currently, no therapeutic exists that effectively and specifically targets hypoxic cells in tumors. Development of such therapeutics critically depends on the availability of in vitro models that accurately recapitulate hypoxia as found in the tumor microenvironment. Here, we report on the design and validation of an easy-to-fabricate tumor-on-a-chip microfluidic platform that robustly emulates the hypoxic tumor microenvironment. The tumor-on-a-chip model consists of a central chamber for 3D tumor cell culture and two side channels for medium perfusion. The microfluidic device is fabricated from polydimethylsiloxane (PDMS), and oxygen diffusion in the device is blocked by an embedded sheet of polymethyl methacrylat

SUBMITTER: Palacio-Castaneda V 

PROVIDER: S-EPMC7231186 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature