Chemotactic Responses of Jurkat Cells in Microfluidic Flow-Free Gradient Chambers.

ABSTRACT: Gradients of soluble molecules coordinate cellular communication in a diverse range of multicellular systems. Chemokine-driven chemotaxis is a key orchestrator of cell movement during organ development, immune response and cancer progression. Chemotaxis assays capable of examining cell responses to different chemokines in the context of various extracellular matrices will be crucial to characterize directed cell motion in conditions which mimic whole tissue conditions. Here, a microfluidic device which can generate different chemokine patterns in flow-free gradient chambers while controlling surface extracellular matrix (ECM) to study chemotaxis either at the population level or at the single cell level with high resolution imaging is presented. The device is produced by combining additive

SUBMITTER: Sonmez UM 

PROVIDER: S-EPMC7231302 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature