Human Organic Anion Transporting Polypeptide 1B3 Applied as an MRI-Based Reporter Gene.

ABSTRACT:

Objective

Recent innovations in biology are boosting gene and cell therapy, but monitoring the response to these treatments is difficult. The purpose of this study was to find an MRI-reporter gene that can be used to monitor gene or cell therapy and that can be delivered without a viral vector, as viral vector delivery methods can result in long-term complications.

Materials and methods

CMV promoter-human organic anion transporting polypeptide 1B3 (CMV-hOATP1B3) cDNA or CMV-blank DNA (control) was transfected into HEK293 cells using Lipofectamine. OATP1B3 expression was confirmed by western blotting and confocal microscopy. In vitro cell phantoms were made using transfected HEK293 cells cultured in various concentrations of gadoxetic acid f

SUBMITTER: Baek SE 

PROVIDER: S-EPMC7231618 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature