Excess dNTPs Trigger Oscillatory Surface Flow in the Early Drosophila Embryo.

ABSTRACT: During the first 2 hours of Drosophila development, precisely orchestrated nuclear cleavages, cytoskeletal rearrangements, and directed membrane growth lead to the formation of an epithelial sheet around the yolk. The newly formed epithelium remains relatively quiescent during the next hour as it is patterned by maternal inductive signals and zygotic gene products. We discovered that this mechanically quiet period is disrupted in embryos with high levels of dNTPs, which have been recently shown to cause abnormally fast nuclear cleavages and interfere with zygotic transcription. High levels of dNTPs are associated with robust onset of oscillatory two-dimensional flows during the third hour of development. Tissue cartography, particle image velocimetry, and dimensionality reduction technique

SUBMITTER: Dutta S 

PROVIDER: S-EPMC7231883 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature