Differentiating Luminal and Membrane-Associated Nuclear Envelope Proteins.

ABSTRACT: The nuclear envelope (NE) consists of two concentric nuclear membranes separated by the lumen, an ∼40-nm-wide fluid layer. NE proteins are implicated in important cellular processes ranging from gene expression to nuclear positioning. Although recent progress has been achieved in quantifying the assembly states of NE proteins in their native environment with fluorescence fluctuation spectroscopy, these studies raised questions regarding the association of NE proteins with nuclear membranes during the assembly process. Monitoring the interaction of proteins with membranes is important because the binding event is often associated with conformational changes that are critical to cellular signaling pathways. Unfortunately, the close physical proximity of both membranes poses a severe experime

SUBMITTER: Hennen J 

PROVIDER: S-EPMC7231917 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature