Dataset Information

Loss of salt tolerance during tomato domestication conferred by variation in a Na⁺ /K⁺ transporter.

ABSTRACT: Domestication has resulted in reduced salt tolerance in tomato. To identify the genetic components causing this deficiency, we performed a genome-wide association study (GWAS) for root Na⁺ /K⁺ ratio in a population consisting of 369 tomato accessions with large natural variations. The most significant variations associated with root Na⁺ /K⁺ ratio were identified within the gene SlHAK20 encoding a member of the clade IV HAK/KUP/KT transporters. We further found that SlHAK20 transports Na⁺ and K⁺ and regulates Na⁺ and K⁺ homeostasis under salt stress conditions. A variation in the coding sequence of SlHAK20 was found to be the causative variant associated with Na⁺ /K⁺ ratio and confer salt tolerance in tomato. Knockout mutations in tomato SlHAK20 and the rice homologous genes resulted in hypersensitivity to salt stress. Together, our study uncovered a previously unknown molecular mechanism of salt tolerance responsible for the deficiency in salt tolerance in cultivated tomato varieties. Our findings provide critical information for molecular breeding to improve salt tolerance in tomato and other crops.

SUBMITTER: Wang Z

PROVIDER: S-EPMC7232006 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Loss of salt tolerance during tomato domestication conferred by variation in a Na+ /K+ transporter.

Wang Zhen Z Hong Yechun Y Zhu Guangtao G Li Yumei Y Niu Qingfeng Q Yao Juanjuan J Hua Kai K Bai Jinjuan J Zhu Yingfang Y Shi Huazhong H Huang Sanwen S Zhu Jian-Kang JK

The EMBO journal 20200305 10

Domestication has resulted in reduced salt tolerance in tomato. To identify the genetic components causing this deficiency, we performed a genome-wide association study (GWAS) for root Na+ /K+ ratio in a population consisting of 369 tomato accessions with large natural variations. The most significant variations associated with root Na+ /K+ ratio were identified within the gene SlHAK20 encoding a member of the clade IV HAK/KUP/KT transporters. We furth ...[more]

PMID: 32134151

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Project description:BackgroundSoil salinization is one of the most crucial abiotic stresses that limit the growth and production of eggplant. The existing researches in eggplant were mostly focused on salt-induced morphological, biochemical and physiological changes, with only limited works centered on salt-response genes in eggplant at the transcriptomic level.ResultsOur preliminary work found that Zhusiqie (No.118) is salt-tolerant and Hongqie (No.30) is salt-sensitive. Consequently, they were re-named as ST118 and SS30, respectively. ST118 showed less damaged on growth and higher K+/Na+ ratios in leaves than SS30. Comparative-transcriptome analysis was used as a powerful approach to understand the salt-response mechanisms in the leaves and roots of SS30 and ST118. And it revealed that genotype-specific and organ-specific manners exist in eggplant in response to salt stress. Strikingly, the genotype-specific differentially expressed genes (DEGs) in ST118 were considered crucial to its higher salt-tolerance, because the expression patterns of common DEGs in the leaves/roots of the two eggplant genotypes were almost the same. Among them, some transcription factors have been reported to be in response to elevated external salinity, including the members of C2C2-CO-like, WRKY, MYB and NAC family. In addition, the AKT1, KAT1 and SOS1 were up-regulated only in the leaves of ST118. Furthermore, the complementation assays demonstrated that the salt-tolerances of both yeast and Arabidopsis akt1 mutants were enhanced by heterologous expression of SmAKT1.ConclusionThe comparative-transcriptome analysis indicated that the salt-tolerance can be increased by higher transcript level of some genotype-specific genes. This work revealed that eggplants seem to be more inclined to absorb K+ rather than to exclude Na+ under salt stress conditions because seven K+ transporters were significantly up-regulated, while only one Na+ transporter was similarly regulated. Finally, the complementation assays of SmAKT1, which is genotype-specific up-regulated in ST118, suggest that the other TFs and K+ transport genes were worthy of future investigation for their functions in salinity tolerance.

Dataset Information

Loss of salt tolerance during tomato domestication conferred by variation in a Na⁺ /K⁺ transporter.

Publications

Loss of salt tolerance during tomato domestication conferred by variation in a Na<sup>+</sup> /K<sup>+</sup> transporter.

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