An Atypical Mechanism of Split Intein Molecular Recognition and Folding.

ABSTRACT: Split inteins associate to trigger protein splicing in trans, a post-translational modification in which protein sequences fused to the intein pair are ligated together in a traceless manner. Recently, a family of naturally split inteins has been identified that is split at a noncanonical location in the primary sequence. These atypically split inteins show considerable promise in protein engineering applications; however, the mechanism by which they associate is unclear and must be different from that of previously characterized canonically split inteins due to unique topological restrictions. Here, we use a consensus design strategy to generate an atypical split intein pair (Cat) that has greatly improved activity and is amenable to detailed biochemical and biophysical analysis. Guided b

SUBMITTER: Stevens AJ 

PROVIDER: S-EPMC7232844 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature