Development of Ratiometric Bioluminescent Sensors for in Vivo Detection of Bacterial Signaling.

ABSTRACT: Second messenger signaling networks allow cells to sense and adapt to changing environmental conditions. In bacteria, the nearly ubiquitous second messenger molecule cyclic di-GMP coordinates diverse processes such as motility, biofilm formation, and virulence. In bacterial pathogens, these signaling networks allow the bacteria to survive changing environmental conditions that are experienced during infection of a mammalian host. While studies have examined the effects of cyclic di-GMP levels on virulence in these pathogens, it has not been possible to visualize cyclic di-GMP levels in real time during the stages of host infection. Toward this goal, we generate the first ratiometric, chemiluminescent biosensor scaffold that selectively responds to c-di-GMP. By engineering the biosensor sca

SUBMITTER: Dippel AB 

PROVIDER: S-EPMC7233196 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature