Application of High Resolution Melt analysis (HRM) for screening haplotype variation in a non-model plant genus: Cyclopia (Honeybush).

ABSTRACT:

Aim

This study has three broad aims: to (a) develop genus-specific primers for High Resolution Melt analysis (HRM) of members of Cyclopia Vent., (b) test the haplotype discrimination of HRM compared to Sanger sequencing, and (c) provide an example of using HRM to detect novel haplotype variation in wild C. subternata Vogel. populations.

Location

The Cape Floristic Region (CFR), located along the southern Cape of South Africa.

Methods

Polymorphic loci were detected through a screening process of sequencing 12 non-coding chloroplast DNA segments across 14 Cyclopia species. Twelve genus-specific primer combinations were designed around variable cpDNA loci, four of which failed to amplify under PCR; the eight remaining were applied to test the specificity, sensitiv

SUBMITTER: Galuszynski NC 

PROVIDER: S-EPMC7233275 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature