Evaluation of two methods to isolate Schwann cells from murine sciatic nerve.

ABSTRACT:

Background

Schwann cells (SC) and macrophages play key roles in the response to peripheral nerve injury (PNI). Accurate isolation of such cells is essential for further analyses that can lead to better understanding of the repair process after PNI. Separation of live SC from the injury site without culture enrichment is necessary for targeted gene expression analysis.

New methods

Two flow cytometric techniques are presented for rapid enrichment of live SC and macrophages from injured murine peripheral nerve without the need for culture.

Results

SC were isolated by fluorescent activated cell sorting (FACS) using transgenic expression of eGFP in SC, or by exclusion of other cell types collected from the injury site.

Comparison with existing method(s)

Gene express

SUBMITTER: Tomlinson JE 

PROVIDER: S-EPMC7233293 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature