Mycelial biomass estimation and metabolic quotient of Lentinula edodes using species-specific qPCR.

ABSTRACT: Lentinula edodes, commonly known as shiitake, is an edible mushroom that is cultivated and consumed around the globe, especially in Asia. Monitoring mycelial growth inside a woody substrate is difficult, but it is essential for effective management of mushroom cultivation. Mycelial biomass also affects the rate of wood decomposition under natural conditions and must be known to determine the metabolic quotient, an important ecophysiological parameter of fungal growth. Therefore, developing a method to measure it inside a substrate would be very useful. In this study, as the first step in understanding species-specific rates of fungal decomposition of wood, we developed species-specific primers and qPCR procedures for L. edodes. We tested primer specificity using strains of L. edodes from J

SUBMITTER: Jomura M 

PROVIDER: S-EPMC7233531 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature