Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding.

ABSTRACT: Fluorescent barcoding is a pivotal technique for the investigation of the microscale world, from information storage to the monitoring of dynamic biochemical processes. Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. However, the use of fluorescence lifetime in this area has been limited by the lack of strategies that provide spatiotemporal manipulation of the coding process. In this study, we design a two-component photo-switchable nanogel that exhibits variable fluorescence lifetime upon photoisomerization-induced energy transfer processes through light irradiation. This remotely manipulated fluorescence lifetime property

SUBMITTER: Xie Y 

PROVIDER: S-EPMC7235003 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature