Pharmacological and genetic characterisation of the canine P2X4 receptor.

ABSTRACT:

Background and purpose

P2X4 receptors are emerging therapeutic targets for treating chronic pain and cardiovascular disease. Dogs are well-recognised natural models of human disease, but information regarding P2X4 receptors in dogs is lacking. To aid the development and validation of P2X4 receptor ligands, we have characterised and compared canine and human P2X4 receptors.

Experimental approach

Genomic DNA was extracted from whole blood samples from 101 randomly selected dogs and sequenced across the P2RX4 gene to identify potential missense variants. Recombinant canine and human P2X4 receptors tagged with Emerald GFP were expressed in 1321N1 and HEK293 cells and analysed by immunoblotting and confocal microscopy. In these cells, receptor pharmacology was characterised using

SUBMITTER: Sophocleous RA 

PROVIDER: S-EPMC7236074 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature