Functional expression and pharmacological modulation of TRPM3 in human sensory neurons.

ABSTRACT:

Background and purpose

The transient receptor potential (TRP) ion channel TRPM3 functions as a noxious heat sensor, plays a key role in acute pain sensation and inflammatory hyperalgesia in rodents. Despite its potential as a novel analgesic drug target, little is known about the expression, function and modulation in the humans.

Experimental approach

We studied TRPM3 in freshly isolated human dorsal root ganglion (hDRG) neurons and human stem cell-derived sensory (hSCDS) neurons. Expression was analysed at the mRNA level using RT-qPCR. Channel function was assessed using Fura-2-based calcium imaging and whole-cell patch-clamp recordings.

Key results

TRPM3 was detected at the mRNA level in both hDRG and hSCDS neurons. The TRPM3 agonists pregnenolone sulphate (PS) and

SUBMITTER: Vangeel L 

PROVIDER: S-EPMC7236075 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature