A novel gene expression system for Ralstonia eutropha based on the T7 promoter.

ABSTRACT:

Background

Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performance of the expression-inducing systems is still limited. In addition, the sizes of the cloned genes are limited due to the large sizes of the plasmid backbones.

Results

In this study, an R. eutropha T7 expression system was established by integrating a T7 RNA polymerase gene driven by the P_BAD promoter into the genome of R. eutropha, as well as adding a T7 promoter into a pBBR1-derived plasmid for gene expression. In addition, the essential DNA sequenc

SUBMITTER: Hu M 

PROVIDER: S-EPMC7236105 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature