Metabolic engineering of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for photoautotrophic production of succinic acid.

ABSTRACT:

Background

Cyanobacteria, a group of photosynthetic prokaryotes, are being increasingly explored for direct conversion of carbon dioxide to useful chemicals. However, efforts to engineer these photoautotrophs have resulted in low product titers. This may be ascribed to the bottlenecks in metabolic pathways, which need to be identified for rational engineering. We engineered the recently reported, fast-growing and robust cyanobacterium, Synechococcus elongatus PCC 11801 to produce succinate, an important platform chemical. Previously, engineering of the model cyanobacterium S. elongatus PCC 7942 has resulted in succinate titer of 0.43 g l^-1 in 8 days.

Results

Building on the previous report, expression of α-ketoglutarate decarboxylase, succinate semia

SUBMITTER: Sengupta S 

PROVIDER: S-EPMC7236211 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature