Integrated single-cell analysis of multicellular immune dynamics during hyperacute HIV-1 infection.

ABSTRACT: Cellular immunity is critical for controlling intracellular pathogens, but individual cellular dynamics and cell-cell cooperativity in evolving human immune responses remain poorly understood. Single-cell RNA-sequencing (scRNA-seq) represents a powerful tool for dissecting complex multicellular behaviors in health and disease^1,2 and nominating testable therapeutic targets³. Its application to longitudinal samples could afford an opportunity to uncover cellular factors associated with the evolution of disease progression without potentially confounding inter-individual variability⁴. Here, we present an experimental and computational methodology that uses scRNA-seq to characterize dynamic cellular programs and their molecular drivers, and apply it to HIV infe

SUBMITTER: Kazer SW 

PROVIDER: S-EPMC7237067 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature