Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m⁵U formation in both tRNA and rRNA.

ABSTRACT: RNA molecules of all species contain modified nucleotides and particularly m⁵U residues. The vertebrate mitochondrial small subunit rRNA contains m⁵U nucleotide in a unique site. In this work we found an enzyme, TRMT2B, responsible for the formation of this nucleotide and m⁵U residues in a number of mitochondrial tRNA species. Inactivation of the Trmt2B gene leads to a reduction of the activity of respiratory chain complexes I, III and IV, containing the subunits synthesized by the mitochondrial protein synthesis apparatus. Comparative sequence analysis of m⁵U-specific RNA methyltransferases revealed an unusual evolutionary pathway of TRMT2B formation which includes consecutive substrate specificity switches from the large subunit rRNA to

SUBMITTER: Laptev I 

PROVIDER: S-EPMC7237156 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature