Dataset Information

Assessment of a pro-healing stent in an animal model of early neoatherosclerosis.

ABSTRACT:

Background

Neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DES in atherosclerotic rabbits.

Methods and results

Neointimal foam cell formation was induced in rabbits (n?=?7). Enhanced endothelial integrity in RGD-coated stents resulted in decreased vascular permeability relative to DES, which was further confirmed by SEM and TEM. Cell culture experiments examined the effect of everolimus on endothelial integrity. Increasing concentrations of everolimus resulted in a dose-dependent decrease of endothelial cell junctions and foam cell transformation of monocytes, confirming the relevance of endothelial integrity in preventing permeability of LDL.

Conclusion

Incomplete endothelial integrity was confirmed as a key factor of neointimal foam cell formation following stent implantation. Pro-healing stent coatings may facilitate reendothelialization and reduce the risk of neoatherosclerosis.

SUBMITTER: Nicol P

PROVIDER: S-EPMC7237429 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Publications

Assessment of a pro-healing stent in an animal model of early neoatherosclerosis.

Nicol Philipp P Lutter Christoph C Bulin Anna A Castellanos Maria Isabel MI Lenz Tobias T Hoppmann Petra P Lahmann Anna Lena AL Colleran Roisin R Euller Kristina K Steigerwald Kristin K Neubauer Stefanie S Rechenmacher Florian F Ludwig Beatrice Stefanie BS Weinmüller Michael M Kerch Garry G Guo Liang L Cheng Qi Q Acampado Eduardo E Koppara Tobias T Kessler Horst H Joner Michael M

Scientific reports 20200519 1

<h4>Background</h4>Neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DES in atherosclerotic rabbits.<h4>Methods and results</h4>Neointimal foam cell formation was induced in rabbits (n = 7). Enhanced endothelial integrity in RGD ...[more]

PMID: 32427835

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Project description:Cardiovascular stent technologies have significantly improved over time. However, their optimal performance remains limited by restenosis, thrombosis, inflammation, and delayed re-endothelialization. Current stent designs primarily target inhibition of neointimal proliferation but do not promote functional arterial healing (pro-healing) in order to restore normal vascular reactivity. The endothelial lining that does develop with current stents appears to have loose intracellular junctions. We have developed a pro-healing nanomatrix coating for stents that enhances healing while limiting neointimal proliferation. This builds on our prior work evaluating the effects of the pro-healing nanomatrix coating on cultures of vascular endothelial cells (ECs), smooth muscle cells (SMCs), monocytes, and platelets. However, when a stent is deployed in an artery, multiple vascular cell types interact, and their interactions affect stent performance. Thus, in our current study, an in vitro vascular double-layer (VDL) system was used to observe stent effects on communication between different vascular cell types. Additionally, we assessed the pro-healing ability and vascular cell interactions after stent deployment in the VDL system and in a rabbit model, evaluating the nanomatrix-coated stent compared to a commercial bare metal stent (BMS) and a drug eluting stent (DES). In vitro results indicated that, in a layered vascular structure, the pro-healing nanomatrix-coated stent could (1) improve endothelialization and endothelial functions, (2) regulate SMC phenotype to reduce SMC proliferation and migration, (3) suppress inflammation through a multifactorial manner, and (4) reduce foam cell formation, extracellular matrix remodeling, and calcification. Consistent with this, in vivo results demonstrated that, compared with commercial BMS and DES, this pro-healing nanomatrix-coated stent enhanced re-endothelialization with negligible restenosis, inflammation, or thrombosis. Thus, these findings indicate the unique pro-healing features of this nanomatrix stent coating with superior efficacy over commercial BMS and DES.

Dataset Information

Assessment of a pro-healing stent in an animal model of early neoatherosclerosis.

Background

Methods and results

Conclusion

Publications

Assessment of a pro-healing stent in an animal model of early neoatherosclerosis.

Similar Datasets

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