Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants.

ABSTRACT: Plastids are morphologically and functionally diverse organelles that are dependent on nuclear-encoded, plastid-targeted proteins for all biochemical and regulatory functions. However, how plastid proteomes vary temporally, spatially, and taxonomically has been historically difficult to analyze at a genome-wide scale using experimental methods. A bioinformatics workflow was developed and evaluated using a combination of fast and user-friendly subcellular prediction programs to maximize performance and accuracy for chloroplast transit peptides and demonstrate this technique on the predicted proteomes of 15 sequenced plant genomes. Gene family grouping was then performed in parallel using modified approaches of reciprocal best BLAST hits (RBH) and UCLUST. A total of 628 protein families were

SUBMITTER: Christian RW 

PROVIDER: S-EPMC7237471 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature