Dissecting the cellular specificity of smoking effects and reconstructing lineages in the human airway epithelium.

ABSTRACT: Cigarette smoke first interacts with the lung through the cellularly diverse airway epithelium and goes on to drive development of most chronic lung diseases. Here, through single cell RNA-sequencing analysis of the tracheal epithelium from smokers and non-smokers, we generate a comprehensive atlas of epithelial cell types and states, connect these into lineages, and define cell-specific responses to smoking. Our analysis infers multi-state lineages that develop into surface mucus secretory and ciliated cells and then contrasts these to the unique specification of submucosal gland (SMG) cells. Accompanying knockout studies reveal that tuft-like cells are the likely progenitor of both pulmonary neuroendocrine cells and CFTR-rich ionocytes. Our smoking analysis finds that all cell types, inc

SUBMITTER: Goldfarbmuren KC 

PROVIDER: S-EPMC7237663 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature