Robust and automated detection of subcellular morphological motifs in 3D microscopy images.

ABSTRACT: Rapid developments in live-cell three-dimensional (3D) microscopy enable imaging of cell morphology and signaling with unprecedented detail. However, tools to systematically measure and visualize the intricate relationships between intracellular signaling, cytoskeletal organization and downstream cell morphological outputs do not exist. Here, we introduce u-shape3D, a computer graphics and machine-learning pipeline to probe molecular mechanisms underlying 3D cell morphogenesis and to test the intriguing possibility that morphogenesis itself affects intracellular signaling. We demonstrate a generic morphological motif detector that automatically finds lamellipodia, filopodia, blebs and other motifs. Combining motif detection with molecular localization, we measure the differential associati

SUBMITTER: Driscoll MK 

PROVIDER: S-EPMC7238333 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature