A novel role of vitronectin in promoting survival of mesenchymal stem cells under serum deprivation stress.

ABSTRACT:

Background

Due to their immunomodulatory and trophic support functions, mesenchymal stem cells (MSCs) are promising in the field of cell-based regenerative medicine. However, MSC survival post-transplantation is challenged by various microenvironment stress factors. Here, we investigated the role of vitronectin (VTN) in the survival strategy of MSCs under serum deprivation stress condition.

Methods

Proliferation kinetics and cell adhesion of MSCs under serum deprivation were determined from population doublings and cell-matrix de-adhesion studies, respectively. mRNA and protein expression levels of VTN were confirmed by qRT-PCR and Western blotting, respectively. Immunofluorescence technique revealed distribution of VTN under serum deprivation stress. siRNA and inhibitor-bas

SUBMITTER: Goyal U 

PROVIDER: S-EPMC7238575 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature