Genome-wide transcriptome analysis reveals equine embryonic stem cell-derived tenocytes resemble fetal, not adult tenocytes.

ABSTRACT:

Background

Tendon injuries occur frequently in human and equine athletes. Treatment options are limited, and the prognosis is often poor with functionally deficient scar tissue resulting. Fetal tendon injuries in contrast are capable of healing without forming scar tissue. Embryonic stem cells (ESCs) may provide a potential cellular therapeutic to improve adult tendon regeneration; however, whether they can mimic the properties of fetal tenocytes is unknown. To this end, understanding the unique expression profile of normal adult and fetal tenocytes is crucial to allow validation of ESC-derived tenocytes as a cellular therapeutic.

Methods

Equine adult, fetal and ESC-derived tenocytes were cultured in a three-dimensional environment, with histological, morphological and trans

SUBMITTER: Paterson YZ 

PROVIDER: S-EPMC7238619 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature