High-quality chromosome-scale assembly of the walnut (Juglans regia L.) reference genome.

ABSTRACT:

Background

The release of the first reference genome of walnut (Juglans regia L.) enabled many achievements in the characterization of walnut genetic and functional variation. However, it is highly fragmented, preventing the integration of genetic, transcriptomic, and proteomic information to fully elucidate walnut biological processes.

Findings

Here, we report the new chromosome-scale assembly of the walnut reference genome (Chandler v2.0) obtained by combining Oxford Nanopore long-read sequencing with chromosome conformation capture (Hi-C) technology. Relative to the previous reference genome, the new assembly features an 84.4-fold increase in N50 size, with the 16 chromosomal pseudomolecules assembled and representing 95% of its total length. Using full-length transcripts

SUBMITTER: Marrano A 

PROVIDER: S-EPMC7238675 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature