Methods for mechanical delivery of viral vectors into rhesus monkey brain.

ABSTRACT:

Background

Modern molecular tools make it possible to manipulate neural activity in a reversible and cell-type specific manner. For rhesus monkey research, molecular tools are generally introduced via viral vectors. New instruments designed specifically for use in monkey research are needed to enhance the efficiency and reliability of vector delivery.

New method

A suite of multi-channel injection devices was developed to permit efficient and uniform vector delivery to cortical regions of the monkey brain. Manganese was co-infused with virus to allow rapid post-surgical confirmation of targeting accuracy using MRI. A needle guide was designed to increase the accuracy of sub-cortical targeting using stereotaxic co-ordinates.

Results

The multi-channel injection devices p

SUBMITTER: Fredericks JM 

PROVIDER: S-EPMC7238764 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature