Targeting c-Met on gastric cancer cells through a fully human fab antibody isolated from a large naive phage antibody library.

ABSTRACT:

Purpose

The aberrant Hepatocyte growth factor (HGF)/ mesenchymal-epithelial transition factor (c-Met) signaling pathway in various malignancies and its correlation with tumor invasion and poor prognosis has validated c-Met as a compelling therapeutic target. Up to now, several monoclonal antibodies and small molecule inhibitors targeting c-Met have been introduced with different outcomes, none are yet clinically approved. Toward the generation of novel fully human anti-c-Met molecules, we generated a large naïve Fab antibody library using phage display technology, which subsequently screened for novel Fabs against c-Met.

Methods

A phage library, with a functional size of 5.5 × 10¹⁰ individual antibody clones, was prepared using standard protocols and screened for

SUBMITTER: Zarei B 

PROVIDER: S-EPMC7238820 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature