Self-Assembly Behaviors of a Penta-Phenylene Maltoside and Its Application for Membrane Protein Study.

ABSTRACT: We prepared an amphiphile with a penta-phenylene lipophilic group and a branched trimaltoside head group. This new agent, designated penta-phenylene maltoside (PPM), showed a marked tendency to self-assembly into micelles via strong aromatic-aromatic interactions in aqueous media, as evidenced by ¹ H NMR spectroscopy and fluorescence studies. When utilized for membrane protein studies, this new agent was superior to DDM, a gold standard conventional detergent, in stabilizing multiple proteins long term. The ability of this agent to form aromatic-aromatic interactions is likely responsible for enhanced protein stabilization when associated with a target membrane protein.

SUBMITTER: Ehsan M 

PROVIDER: S-EPMC7239035 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature