Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21.

ABSTRACT:

Objectives

Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and secretion. It also has a role in depolymerization of alginates. Using AlgLs in cystic fibrosis patients along with antibiotics enhances bacterial killing and host healing. In this study, we investigated the different biochemical properties of a newly isolated AlgL from P. aeruginosa S21 to complete the databank of AlgLs.

Materials and methods

The enzyme was extracted from the periplasmic space of the bacteria by the heat shock method. Using the TBA method, the enzyme activity and biochemical properties were as

SUBMITTER: Piroozmand F 

PROVIDER: S-EPMC7239423 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature