Stem cell function is conserved during short-term storage of cultured epidermal cell sheets at 12°C.

ABSTRACT: Transplantation of cultured epidermal cell sheets (CES) can be life-saving for patients with large area burns. CES have also been successfully used to regenerate eye and urethral epithelia in animal models. Short-term storage aims to extend the transplantation window, offers flexibility in timing surgery and allows testing of CES quality, phenotype and sterility. This study investigated extended CES storage and explored the effect of additional re-incubation recovery time following storage. The proliferative quality of stored confluent versus pre-confluent CES was also investigated using functional testing. CES were stored at 12°C and results compared to non-stored control CES. Investigation of timepoints during 15 days storage revealed that viability began to deteriorate by day 11 and was

SUBMITTER: Ringstad H 

PROVIDER: S-EPMC7239464 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature