ERK Activity in Immature Leukemic Cells Drives Clonal Selection during Induction Therapy for Acute Myeloid Leukemia.

ABSTRACT: Selection of resistant clones following intensive chemotherapy is a common obstacle for cure in many cancers, particularly in acute myeloid leukemia (AML). In AML, clone-specific sensitivity to chemotherapy varies even within the same patient. Multiple mutations and genetic aberrations are associated with clones surviving chemotherapy. The current study explored the role of activated signaling pathways in chemoresistance as a function of cell maturation, reflected by CD34 expression. In-vitro, Kasumi-1 leukemic cell line, sorted by CD34 expression, showed increased apoptosis only in the CD34^- subpopulation after exposure to cytosine arabinoside (Ara-C) or daunorubicin. The resistant CD34⁺ subset demonstrated higher expression of ERK1/2 and BCL-2 proteins than CD34

SUBMITTER: Hayun M 

PROVIDER: S-EPMC7239856 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature