In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei.

ABSTRACT: Serial passage is a problem among many bacterial species, especially those where strains have been stored (banked) for several decades. Prior to banking with an organization such as ATCC, many bacterial strains were passaged for many years, so the characteristics of each strain may be extremely different. This is in addition to any differences in the original host environment. For Burkholderia pseudomallei, the number of serial passages should be carefully defined for each experiment because it undergoes adaptation during the course of serial passages. In the present study, we found that passaged B. pseudomallei fresh clinical isolates and reference strain in Luria-Bertani broth exhibited increased plaque formation, invasion, intracellular replication, Galleria mellonella killing abilities

SUBMITTER: Duangurai T 

PROVIDER: S-EPMC7239947 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature