A technique for removing tumourigenic pluripotent stem cells using rBC2LCN lectin.

ABSTRACT:

Introduction

Tumourigenesis attributed to residual undifferentiated cells in a graft is considered to be a significant issue in cell therapy using human pluripotent stem cells. To ensure the safety of regenerative medicine derived from pluripotent stem cells, residual undifferentiated cells must be eliminated in the manufacturing process. We previously described the lectin probe rBC2LCN, which binds harmlessly and specifically to the cell surface of human pluripotent stem cells. We report here a technique using rBC2LCN to remove pluripotent cells from a heterogenous population to reduce the chance of teratoma formation.

Methods

We demonstrate a method for separating residual tumourigenic cells using rBC2LCN-bound magnetic beads. This technology is a novel use of their previo

SUBMITTER: Haramoto Y 

PROVIDER: S-EPMC7240284 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature