Prostate-specific antigen modulates the osteogenic differentiation of MSCs via the cadherin 11-Akt axis.

ABSTRACT:

Background

A high prevalence of osteoblastic bone metastases is characteristic of prostate cancer. Prostate-specific antigen (PSA) is a serine protease uniquely produced by prostate cancer cells and is an important serological marker for prostate cancer. However, whether PSA modulates the osteogenic process remains largely unknown. In this study, we explored the effect of PSA on modulating the osteoblastic differentiation of mesenchymal stem cells (MSCs). In this study, we used flow cytometry, CCK-8 assay, Alizarin red S (ARS) staining and quantification, alkaline phosphatase (ALP) activity and staining, Western blotting, and quantitative real-time PCR (qRT-PCR) to explore the effect of PSA on osteogenic differentiation of MSCs.

Results

We first demonstrated that although PS

SUBMITTER: Wu L 

PROVIDER: S-EPMC7240859 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature