Erythrocyte microRNAs show biomarker potential and implicate multiple sclerosis susceptibility genes.

ABSTRACT:

Background

Multiple sclerosis is a demyelinating autoimmune disease, for which there is no blood-borne biomarker. Erythrocytes may provide a source of such biomarkers as they contain microRNAs. MicroRNAs regulate protein translation through complementary binding to messenger RNA. As erythrocytes are transcriptionally inactive, their microRNA profiles may be less susceptible to variation. The aim of this study was to assess the biomarker potential of erythrocyte microRNAs for multiple sclerosis and assess the potential contribution of erythrocyte-derived extracellular vesicle microRNAs to pathology.

Methods

Erythrocytes were isolated from whole blood by density gradient centrifugation. Erythrocyte microRNAs of a discovery cohort (23 multiple sclerosis patients and 22 healthy

SUBMITTER: Groen K 

PROVIDER: S-EPMC7240864 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature