Statistical differences resulting from selection of stable reference genes after hypoxia and hypothermia in the neonatal rat brain.

ABSTRACT: Real-time reverse transcription PCR (qPCR) normalized to an internal reference gene (RG), is a frequently used method for quantifying gene expression changes in neuroscience. Although RG expression is assumed to be constant independent of physiological or experimental conditions, several studies have shown that commonly used RGs are not expressed stably. The use of unstable RGs has a profound effect on the conclusions drawn from studies on gene expression, and almost universally results in spurious estimation of target gene expression. Approaches aimed at selecting and validating RGs often make use of different statistical methods, which may lead to conflicting results. Based on published RG validation studies involving hypoxia the present study evaluates the expression of 5 candidate RGs

SUBMITTER: Bustelo M 

PROVIDER: S-EPMC7241816 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature