ALC1/eIF4A1-mediated regulation of CtIP mRNA stability controls DNA end resection.

ABSTRACT: During repair of DNA double-strand breaks, resection of DNA ends influences how these lesions will be repaired. If resection is activated, the break will be channeled through homologous recombination; if not, it will be simply ligated using the non-homologous end-joining machinery. Regulation of resection relies greatly on modulating CtIP, which can be done by modifying: i) its interaction partners, ii) its post-translational modifications, or iii) its cellular levels, by regulating transcription, splicing and/or protein stability/degradation. Here, we have analyzed the role of ALC1, a chromatin remodeler previously described as an integral part of the DNA damage response, in resection. Strikingly, we found that ALC1 affects resection independently of chromatin remodeling activity or its a

SUBMITTER: Mejias-Navarro F 

PROVIDER: S-EPMC7241833 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature