Detection of avian influenza virus: a comparative study of the in silico and in vitro performances of current RT-qPCR assays.

ABSTRACT: Avian influenza viruses (AIV) are negative sense RNA viruses posing a major threat to the poultry industry worldwide, with the potential to spread to mammals, including humans; hence, an accurate and rapid AIV diagnosis is essential. To date AIV detection relies on molecular methods, mainly RT-qPCR directed against AIV M gene segment. The evolution of AIV represents a relevant issue in diagnostic RT-qPCR due to possible mispriming and/or probe-binding failures resulting in false negative results. Consequently, RT-qPCR for AIV detection should be periodically re-assessed both in silico and in vitro. To this end, a specific workflow was developed to evaluate in silico the complementarity of primers and probes of four published RT-qPCR protocols to their target regions. The four assays and on

SUBMITTER: Laconi A 

PROVIDER: S-EPMC7242438 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature