Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs.

ABSTRACT: The programmed construction of functional synthetic cells requires spatial control over arrays of biomolecules within the cytomimetic environment. The mimicry of the natural hierarchical assembly of biomolecules remains challenging due to the lack of an appropriate molecular toolbox. Herein, we report the implementation of DNA-decorated supramolecular assemblies as dynamic and responsive nanoscaffolds for the localization of arrays of DNA signal cargo within hierarchically assembled complex coacervate protocells. Protocells stabilized with a semipermeable membrane allow trafficking of single-stranded DNA between neighboring protocells. DNA duplex operations demonstrate the responsiveness of the nanoscaffolds to different input DNA strands via the reversible release of DNA cargo. Moreover,

SUBMITTER: Magdalena Estirado E 

PROVIDER: S-EPMC7243252 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature