In vivo chromatin organization on native yeast telomeric regions is independent of a cis-telomere loopback conformation.

ABSTRACT:

Background

DNA packaging into chromatin regulates all DNA-related processes and at chromosomal ends could affect both essential functions of telomeres: protection against DNA damage response and telomere replication. Despite this primordial role of chromatin, little is known about chromatin organization, and in particular about nucleosome positioning on unmodified subtelomere-telomere junctions in Saccharomyces cerevisiae.

Results

By ChEC experiments and indirect end-labeling, we characterized nucleosome positioning as well as specialized protein-DNA associations on most subtelomere-telomere junctions present in budding yeast. The results show that there is a relatively large nucleosome-free region at chromosome ends. Despite the absence of sequence homologies between the tw

SUBMITTER: Pasquier E 

PROVIDER: S-EPMC7243337 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature