A Confocal Reflection Super-Resolution Technique to Image Golgi-Cox Stained Neurons.

ABSTRACT: Metal-based Golgi-Cox (GC) staining is an established method used to visualise neurons with great morphological detail. Although GC stained samples are imaged routinely under transmitted light microscopy, this method is unable to yield information on the three-dimensional structure of dendrites and neurons and thus help reveal the connective properties of the central nervous system. Although a few studies have attempted simultaneous visualisation of GC staining and antigen-specific fluorescent labelling under a confocal reflection technique, the resolution of both confocal reflection and fluorescence modalities used to acquire GC reflection and fluorescently stained antibody signals are still limited by the diffraction limit of light at about 220 nm. Here, we report a confocal reflection s

SUBMITTER: Sivaguru M 

PROVIDER: S-EPMC7243675 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature