EZcalcium: Open-Source Toolbox for Analysis of Calcium Imaging Data.

ABSTRACT: Fluorescence calcium imaging using a range of microscopy approaches, such as two-photon excitation or head-mounted "miniscopes," is one of the preferred methods to record neuronal activity and glial signals in various experimental settings, including acute brain slices, brain organoids, and behaving animals. Because changes in the fluorescence intensity of genetically encoded or chemical calcium indicators correlate with action potential firing in neurons, data analysis is based on inferring such spiking from changes in pixel intensity values across time within different regions of interest. However, the algorithms necessary to extract biologically relevant information from these fluorescent signals are complex and require significant expertise in programming to develop robust analysis pip

SUBMITTER: Cantu DA 

PROVIDER: S-EPMC7244005 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature