Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation.

ABSTRACT: In response to injury, adult Schwann cells (SCs) re-enter the cell cycle, change their expression profile, and exert repair functions important for wound healing and the re-growth of axons. While this phenotypical instability of SCs is essential for nerve regeneration, it has also been implicated in cancer progression and de-myelinating neuropathies. Thus, SCs became an important research tool to study the molecular mechanisms involved in repair and disease and to identify targets for therapeutic intervention. A high purity of isolated SC cultures used for experimentation must be demonstrated to exclude that novel findings are derived from a contaminating fibroblasts population. In addition, information about the SC proliferation status is an important parameter to be determined in respons

SUBMITTER: Weiss T 

PROVIDER: S-EPMC7244157 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature