Dataset Information

Comparison of skeletal and soft tissue pericytes identifies CXCR4⁺ bone forming mural cells in human tissues.

ABSTRACT: Human osteogenic progenitors are not precisely defined, being primarily studied as heterogeneous multipotent cell populations and termed mesenchymal stem cells (MSCs). Notably, select human pericytes can develop into bone-forming osteoblasts. Here, we sought to define the differentiation potential of CD146⁺ human pericytes from skeletal and soft tissue sources, with the underlying goal of defining cell surface markers that typify an osteoblastogenic pericyte. CD146⁺CD31^-CD45^- pericytes were derived by fluorescence-activated cell sorting from human periosteum, adipose, or dermal tissue. Periosteal CD146⁺CD31^-CD45^- cells retained canonical features of pericytes/MSC. Periosteal pericytes demonstrated a striking tendency to undergo osteoblastogenesis in vitro and skeletogenesis in vivo, while soft tissue pericytes did not readily. Transcriptome analysis revealed higher CXCR4 signaling among periosteal pericytes in comparison to their soft tissue counterparts, and CXCR4 chemical inhibition abrogated ectopic ossification by periosteal pericytes. Conversely, enrichment of CXCR4⁺ pericytes or stromal cells identified an osteoblastic/non-adipocytic precursor cell. In sum, human skeletal and soft tissue pericytes differ in their basal abilities to form bone. Diversity exists in soft tissue pericytes, however, and CXCR4⁺ pericytes represent an osteoblastogenic, non-adipocytic cell precursor. Indeed, enrichment for CXCR4-expressing stromal cells is a potential new tactic for skeletal tissue engineering.

SUBMITTER: Xu J

PROVIDER: S-EPMC7244476 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues.

Xu Jiajia J Li Dongqing D Hsu Ching-Yun CY Tian Ye Y Zhang Leititia L Wang Yiyun Y Tower Robert J RJ Chang Leslie L Meyers Carolyn A CA Gao Yongxing Y Broderick Kristen K Morris Carol C Hooper Jody E JE Nimmagadda Sridhar S Péault Bruno B James Aaron W AW

Bone research 20200522 1

Human osteogenic progenitors are not precisely defined, being primarily studied as heterogeneous multipotent cell populations and termed mesenchymal stem cells (MSCs). Notably, select human pericytes can develop into bone-forming osteoblasts. Here, we sought to define the differentiation potential of CD146+ human pericytes from skeletal and soft tissue sources, with the underlying goal of defining cell surface markers that typify an osteoblastogenic pericyte. CD146+CD31<sup ...[more]

PMID: 32509378

Similar Datasets

Project description:ObjectivesTo establish the three-dimensional facial soft tissue morphology of adolescent and adult females in the Guangdong population and to study the morphological characteristics of hyperdivergent skeletal class II females in Guangdong compared with that of normodivergent class I groups.Materials and methodsThe 3dMDface system was used to capture face scans of 160 patients, including 45 normal and 35 hyperdivergent skeletal class II adolescents (aged 11-14 years old) and 45 normal and 35 hyperdivergent skeletal class II adults (aged 18-30 years old). Thirty-two soft tissue landmarks were mapped, and 21 linear, 10 angular and 17 ratio measurements were obtained by 3dMDvultus analysis software. Data were assessed with a t-test of two independent samples between the normal adolescent and adult groups and between the normal and hyperdivergent skeletal class II groups.ResultsThe linear measurements of the Guangdong adult females were larger than those of the adolescents in both Class I and Class II groups. However, the angular and ratio measurements had no significant difference. The vertical linear measurements were higher and the sagittal and transverse linear measurements were smaller in the hyperdivergent class II group (p < 0.05). The soft tissue ANB angle, chin-lip angle, and mandibular angle were significantly larger and the soft tissue facial convexity angle and nasal convexity angle were significantly smaller in the hyperdivergent class II group (p < 0.05). Additionally, there were significant differences in the ratio measurements between the hyperdivergent class II groups and the control groups (p < 0.05).ConclusionsThe three-dimensional facial morphology of Guangdong adolescent and adult females was acquired. The facial soft tissue measurements of the adults were higher in the three dimensions except for the facial convexity and proportional relationships which were similar, suggesting that the growth pattern remained the same. The three-dimensional facial soft tissue features of hyperdivergent skeletal class II were characterized by the terms "long, convex, and narrow". Three-dimensional facial measurements can reflect intrinsic hard tissue characteristics.

Dataset Information

Comparison of skeletal and soft tissue pericytes identifies CXCR4⁺ bone forming mural cells in human tissues.

Publications

Comparison of skeletal and soft tissue pericytes identifies CXCR4<sup>+</sup> bone forming mural cells in human tissues.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues.

Publications

Comparison of skeletal and soft tissue pericytes identifies CXCR4&lt;sup&gt;+&lt;/sup&gt; bone forming mural cells in human tissues.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Comparison of skeletal and soft tissue pericytes identifies CXCR4⁺ bone forming mural cells in human tissues.

Comparison of skeletal and soft tissue pericytes identifies CXCR4<sup>+</sup> bone forming mural cells in human tissues.