Dataset Information

The high efficacy of luliconazole against environmental and otomycosis Aspergillus flavus strains.

ABSTRACT:

Background and objectives

Luliconazole is currently confirmed for the topical therapy of dermatophytosis. Moreover, it is found that luliconazole has in vitro activity against some molds and yeast species. The aim of the present study was to evaluate the efficacy of luliconazole in comparison to routine used antifungals on clinical and environmental isolates of Aspergillus flavus.

Materials and methods

Thirty eight isolates of A. flavus (18 environmental and 20 clinical isolates) were detected based on morphological and microscopic features and also PCR-sequencing of β-tubulin ribosomal DNA gene. All the isolates were tested against luliconazole, voriconazole, amphotericin B and caspofungin. Minimum inhibitory concentration (MIC), MIC₅₀, MIC₉₀ and MIC Geometric (GM) were calculated using CLSI M38-A2 protocol for both environmental and clinical isolates.

Results

Luliconazole with extremely low MIC range, 0.00049-0.00781 μg/mL and MIC_GM 0.00288 μg/mL showed very strong activity against both clinical and environmental A. flavus isolates. Moreover, voriconazole inhibited 100% of isolates at defined epidemiological cutoff values (ECV ≤ 2 μg/ml). 50% and 27.8% of clinical and environmental isolates of A. flavus, were resistant to caspofungin, respectively. Whereas, all the isolates were found to be resistant to amphotericin B.

Conclusion

The analysis of our data clearly indicated that luliconazole (with MIC_GM 0.00244 μg/ml for clinical and 0.00336 μg/ml for environmental isolates) had the highest in vitro activity against A. flavus strains.

SUBMITTER: Moslem M

PROVIDER: S-EPMC7244823 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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The high efficacy of luliconazole against environmental and otomycosis <i>Aspergillus flavus</i> strains.

Moslem Maryam M Mahmoudabadi Ali Zarei AZ

Iranian journal of microbiology 20200401 2

<h4>Background and objectives</h4>Luliconazole is currently confirmed for the topical therapy of dermatophytosis. Moreover, it is found that luliconazole has <i>in vitro</i> activity against some molds and yeast species. The aim of the present study was to evaluate the efficacy of luliconazole in comparison to routine used antifungals on clinical and environmental isolates of <i>Aspergillus flavus</i>.<h4>Materials and methods</h4>Thirty eight isolates of <i>A. flavus</i> (18 environmental and 2 ...[more]

PMID: 32494352

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Project description:Background and Objectives:Black Aspergillus strains including, Aspergillus niger and A. tubingensis, are the most cause of otomycosis with worldwide distribution. Although, amphotericin B was a Gold standard for the treatment of invasive fungal infection for several decades, it gradually replaced by fluconazole and /or voriconazole. Moreover, luliconazole, appears to offer the best potential for in vitro activity against black Aspergillus strains. The aim of the present study was to compare the in vitro activity luliconazole, with commonly used antifungals against clinical and environmental strains of black Aspergillus. Materials and Methods:Sixty seven (37 clinical and 30 environmental) strains of black Aspergillus were identified using morphological and molecular technique (?-Tubulin gene). In addition, antifungal susceptibility test was applied according to CLSI M38 A2. The results were reported as minimum inhibitory concentration (MIC) or minimum effective concentration (MEC) range, MIC50 or MEC50, MIC90 or MEC90 and MIC geometric (GM) or MECGM. Results:Aspergillus niger was the common isolate followed by, A. tubingensis in both clinical and environmental strains. The lowest MIC range, MIC50, MIC90, and MICGM was attributed to luliconazole in clinical strains. The highest resistant rate was found in amphotericin B for both clinical (86.5%) and environmental (96.7%) strains whereas 54.1% of clinical and 30% of environmental isolates were resistant to caspofungin. Clinical strains of Aspergillus were more sensitive to voriconazole (86.7%) than environmental strains (70.3%). On the other hand, 83.8% of clinical and 70% of environmental isolates were resistant to posaconazole. Conclusion:Luliconazole versus amphotericin B, voriconazole, posaconazole and caspofungin is a potent antifungal for Aspergillus Nigri complex. The in vitro extremely antifungal efficacy against black Aspergillus strains of luliconazole, is different from those of other used antifungals.