Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells.

ABSTRACT: Advances in gene editing are leading to new medical interventions where patients' own cells are used for stem cell therapies and immunotherapies. One of the key limitations to translating these treatments to the clinic is the need for scalable technologies for engineering cells efficiently and safely. Toward this goal, microfluidic strategies to induce membrane pores and permeability have emerged as promising techniques to deliver biomolecular cargo into cells. As these technologies continue to mature, there is a need to achieve efficient, safe, nontoxic, fast, and economical processing of clinically relevant cell types. We demonstrate an acoustofluidic sonoporation method to deliver plasmids to immortalized and primary human cell types, based on pore formation and permeabilization of cell

SUBMITTER: Belling JN 

PROVIDER: S-EPMC7245081 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature