A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells.

ABSTRACT: Human brain organoids differentiated from embryonic stem cells offer the unique opportunity to study complicated interactions of multiple cell types in a three-dimensional system. Here we present a relatively straightforward and inexpensive method that yields brain organoids. In this protocol human pluripotent stem cells are broken into small clusters instead of single cells and grown in basic media without a heterologous basement membrane matrix or exogenous growth factors, allowing the intrinsic developmental cues to shape the organoid's growth. This simple system produces a diversity of brain cell types including glial and microglial cells, stem cells, and neurons of the forebrain, midbrain, and hindbrain. Organoids generated from this protocol also display hallmarks of appropriate temp

SUBMITTER: Boisvert EM 

PROVIDER: S-EPMC7245934 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature