Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate.

ABSTRACT: The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to increase the biosynthetic product yield. Different clustering strategies have particular advantages as the spatial organization of multiple enzymes creates biocatalytic cascades with a higher efficiency of biochemical reaction. However, there are also some drawbacks, such as misfolding and the variable stability of interaction domains, which may differ between particular biosynthetic reactions and the host organism. Here, we compared different protein-based clustering strategies, including direct fusion, fusion mediated by intein, and noncovalent interactions mediated through small c

SUBMITTER: Fink T 

PROVIDER: S-EPMC7246283 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature